Journal: Molecular Medicine Reports

Article Title: Therapeutic effect of transplantation of human bone marrow-derived mesenchymal stem cells on neuron regeneration in a rat model of middle cerebral artery occlusion

doi: 10.3892/mmr.2019.10536

Figure Lengend Snippet: Expression of BMSC surface markers (CD34, CD44 and CD45) detected by the immunofluorescence assay and the positive rate of CD44 is 92.75%, indicating that most of the cells we isolated are human bone marrow mesenchymal stem cells, conforming to further experimental requirements. (A) CD34, CD44 and CD45, as examined by immunofluorescence assay (magnification, ×400). Green, surface markers; blue, nuclei. (B) Positive expression rates of CD34, CD44 and CD45.

Article Snippet: Next, the cells were incubated at room temperature for 3 h with the following primary antibodies: Rat anti-human CD34 (bsm-16538M, 1:100; BIOSS), CD44 (bsm-51065M, 1:100; BIOSS) and CD45 (bs-4819R, 1:100; BIOSS).

Techniques: Expressing, Immunofluorescence, Isolation

Journal: Cells, tissues, organs

Article Title: Conditioned medium from bone marrow mesenchymal stem cells transiently retards osteoblast differentiation by downregulating runx2.

doi: 10.1159/000339245

Figure Lengend Snippet: Fig. 1. Culture and identification of rat MSCs. a Primary MSCs in culture, 7 days after seeding. b MSCs of pas- sage 3 in culture, 1 day after seeding. c Expression of CD29, CD44, CD90, CD34, CD45, and CD11b by MSCs at passage 3. Numbers indicate the percentages of cells positive for each of the surface markers.

Article Snippet: Appropriate specific antibodies were added (FITC anti-rat CD90, PE antirat CD29, PE anti-rat CD45, and PE anti-rat CD11b/c, all from Biolegend, San Diego, Calif., USA; CD34 antibody from Santa Cruz, Calif., USA; mouse anti-rat CD44 from Serotec, Oxford, UK, isotype-matched controls and blank control).

Techniques: Expressing

Journal: The Tohoku journal of experimental medicine

Article Title: 17β-estradiol induces the proliferation of hematopoietic stem cells by promoting the osteogenic differentiation of mesenchymal stem cells.

doi: 10.1620/tjem.233.141

Figure Lengend Snippet: Fig. 1. Morphological characteristics and cell surface markers of rat MSCs. A. Morphological characteristics of rat MSCs. (a) After incubation for 96 h, most primary MSCs adhered to the surface of the culture dish and were round, although some were irregular and fusiform in shape (arrows); (b) primary adherent cells tended to become fusiform, spindle-shaped (arrows) and more numerous after incubation for 5-7 days. B. Cell sur- face markers of rat MSCs. The cell surface markers are positive for CD44, CD90 and CD105, but negative for CD34 and CD45.

Article Snippet: Each cell suspension (5 × 103 cells/100 μL) was incubated with lineagespecific monoclonal antibodies, such as FITC mouse anti-rat CD29, FITC mouse anti-rat CD90, PE mouse anti-rat CD34, PE mouse antirat CD45 (BD Pharmingen, USA), FITC mouse anti-rat CD44 (SeroTec, USA) and nonspecific mouse IgG (isotype control) for 30 min at 4°C.

Techniques: Incubation

Journal: The Tohoku journal of experimental medicine

Article Title: 17β-estradiol induces the proliferation of hematopoietic stem cells by promoting the osteogenic differentiation of mesenchymal stem cells.

doi: 10.1620/tjem.233.141

Figure Lengend Snippet: Fig. 3. E2 induces the proliferation of HSCs when co-cultured with MSCs in OM. A. The morphology of colony-forming unit-granulocyte/macrophage (CFU-GM) derived from HSCs. B. Number of CFUs-GM derived from HSCs when HSCs were cultured in IMDM, OM, IMDM with MSCs or OM with MSCs at dif- ferent concentrations of E2. Without E2 treatment, the number of CFUs-GM from HSCs cultured in OM with MSCs was significantly higher than from HSCs cultured in IMDM, OM or IMDM with MSCs (*p < 0.05). As the concentra- tion of E2 increased, the difference between these culture systems became more and more significant (p < 0.05).

Article Snippet: Each cell suspension (5 × 103 cells/100 μL) was incubated with lineagespecific monoclonal antibodies, such as FITC mouse anti-rat CD29, FITC mouse anti-rat CD90, PE mouse anti-rat CD34, PE mouse antirat CD45 (BD Pharmingen, USA), FITC mouse anti-rat CD44 (SeroTec, USA) and nonspecific mouse IgG (isotype control) for 30 min at 4°C.

Techniques: Cell Culture, Derivative Assay

Journal: Cells, tissues, organs

Article Title: Conditioned medium from bone marrow mesenchymal stem cells transiently retards osteoblast differentiation by downregulating runx2.

doi: 10.1159/000339245

Figure Lengend Snippet: Fig. 1. Culture and identification of rat MSCs. a Primary MSCs in culture, 7 days after seeding. b MSCs of pas- sage 3 in culture, 1 day after seeding. c Expression of CD29, CD44, CD90, CD34, CD45, and CD11b by MSCs at passage 3. Numbers indicate the percentages of cells positive for each of the surface markers.

Article Snippet: Appropriate specific antibodies were added (FITC anti-rat CD90, PE antirat CD29, PE anti-rat CD45, and PE anti-rat CD11b/c, all from Biolegend, San Diego, Calif., USA; CD34 antibody from Santa Cruz, Calif., USA; mouse anti-rat CD44 from Serotec, Oxford, UK, isotype-matched controls and blank control).

Techniques: Expressing

Journal: Cell Transplantation

Article Title: A novel approach for hepatocyte transplantation at the liver surface

doi: 10.1177/09636897251329308

Figure Lengend Snippet: Characterization of ADSCs. Flow cytometry showed strong expression of CD29 (99.1%), CD44 (98.2%), CD90 (97.4%), and CD105 (98.7%), and moderate expression of CD73 (54.4%). In contrast, markers associated with endothelial cells (CD31) and hematopoietic lineage cells (CD34 and CD45) were not detected.

Article Snippet: ADSCs at passage 3 isolated from F344/NSlc rats were labeled with fluorescein isothiocyanate-conjugated anti-rat CD29 antibody (Becton, Dickinson and Company, Franklin Lakes, NJ, USA), anti-rat CD31 antibody (Thermo Fisher Scientific Inc.), anti-rat CD34 antibody (Bioss, Inc., Woburn, MA, USA), anti-rat CD44 antibody (Becton, Dickinson and Company), anti-rat CD45 antibody (Becton, Dickinson and Company), anti-rat CD73 antibody (Bioss, Inc.), anti-rat CD90 antibody (Becton, Dickinson and Company), and anti-rat CD105 antibody (Bioss, Inc.) for 45 min on ice.

Techniques: Flow Cytometry, Expressing

Journal: Cells, tissues, organs

Article Title: Conditioned medium from bone marrow mesenchymal stem cells transiently retards osteoblast differentiation by downregulating runx2.

doi: 10.1159/000339245

Figure Lengend Snippet: Fig. 1. Culture and identification of rat MSCs. a Primary MSCs in culture, 7 days after seeding. b MSCs of pas- sage 3 in culture, 1 day after seeding. c Expression of CD29, CD44, CD90, CD34, CD45, and CD11b by MSCs at passage 3. Numbers indicate the percentages of cells positive for each of the surface markers.

Article Snippet: Appropriate specific antibodies were added (FITC anti-rat CD90, PE antirat CD29, PE anti-rat CD45, and PE anti-rat CD11b/c, all from Biolegend, San Diego, Calif., USA; CD34 antibody from Santa Cruz, Calif., USA; mouse anti-rat CD44 from Serotec, Oxford, UK, isotype-matched controls and blank control).

Techniques: Expressing